Journal: International Journal of Nanomedicine

Article Title: Atomic layer deposition of nano-TiO₂ thin films with enhanced biocompatibility and antimicrobial activity for orthopedic implants

doi: 10.2147/ijn.s148065

Figure Lengend Snippet: Figure 10 Osteoblast proliferation on Ti and Ti-TiO2 (160°C) samples. Data represent mean ± SD. N=3. *p,0.05 compared with Ti at the same time period, **p,0.05 compared with the same sample (Day 1). Abbreviations: Ti, titanium; TiO2, titanium dioxide.

Article Snippet: Casein protein has been shown to be a key protein in TSB medium (17 g casein/L); thus, to quantify casein protein adsorption, we incubated samples with the prepared 1.7% by weight casein solution (17 g casein/L H 2 O, Sigma-Aldrich), and then the same procedures, as already described, to determine total protein adsorption were used. cell assays cell culture Human osteoblasts (PromoCell, C-12720) and human dermal fibroblasts (Lonza, CC-2509) at population numbers less than 10 were used for all cell experiments.

Techniques: Titanium Dioxide

Journal: International Journal of Nanomedicine

Article Title: Atomic layer deposition of nano-TiO₂ thin films with enhanced biocompatibility and antimicrobial activity for orthopedic implants

doi: 10.2147/ijn.s148065

Figure Lengend Snippet: Figure 9 Osteoblast adhesion on Ti samples with different TiO2 coatings after 4 hours of culture. Data represent mean ± SD, N=3. *p,0.05 compared with Ti control (labeled as Ti). Abbreviations: Ti, titanium; TiO2, titanium dioxide.

Article Snippet: Casein protein has been shown to be a key protein in TSB medium (17 g casein/L); thus, to quantify casein protein adsorption, we incubated samples with the prepared 1.7% by weight casein solution (17 g casein/L H 2 O, Sigma-Aldrich), and then the same procedures, as already described, to determine total protein adsorption were used. cell assays cell culture Human osteoblasts (PromoCell, C-12720) and human dermal fibroblasts (Lonza, CC-2509) at population numbers less than 10 were used for all cell experiments.

Techniques: Control, Labeling, Titanium Dioxide

Journal: International Journal of Nanomedicine

Article Title: Atomic layer deposition of nano-TiO₂ thin films with enhanced biocompatibility and antimicrobial activity for orthopedic implants

doi: 10.2147/ijn.s148065

Figure Lengend Snippet: Figure 12 (A) Osteoblast and (B) fibroblast cell density (after 4 hours of culture) was directly proportional to the surface energy on Ti controls and Ti-TiO2 samples. Error bars represent SD. Abbreviations: Ti, titanium; TiO2, titanium dioxide.

Article Snippet: Casein protein has been shown to be a key protein in TSB medium (17 g casein/L); thus, to quantify casein protein adsorption, we incubated samples with the prepared 1.7% by weight casein solution (17 g casein/L H 2 O, Sigma-Aldrich), and then the same procedures, as already described, to determine total protein adsorption were used. cell assays cell culture Human osteoblasts (PromoCell, C-12720) and human dermal fibroblasts (Lonza, CC-2509) at population numbers less than 10 were used for all cell experiments.

Techniques: Titanium Dioxide

Journal: The Journal of Experimental Medicine

Article Title: Immune regulation by glucocorticoids can be linked to cell type–dependent transcriptional responses

doi: 10.1084/jem.20180595

Figure Lengend Snippet: The direction and magnitude of transcriptional regulation by glucocorticoids are cell type dependent. Four primary human hematopoietic cell types and five primary human nonhematopoietic cell types were studied. For each cell type, cells from four unrelated healthy donors were independently cultured and treated with methylprednisolone (22.7 µM) or vehicle (0.08% ethanol). Total RNA was purified 2 and 6 h after in vitro treatment and RNA-seq was performed. Differential expression was assessed by comparing data from methylprednisolone-treated versus vehicle-treated cells in the four biological replicates. The statistical significance of differential expression was calculated with a Wald test, after accounting for dispersion, library size, and read count. The resulting P values for differential expression were adjusted for multiple testing by the method of . (a) The left panel displays the transcriptional response to glucocorticoids in hematopoietic cells versus nonhematopoietic cells for each of 56,870 genes. The log2 fold change compares methylprednisolone-treated versus vehicle-treated cells after 6 h of in vitro treatment. Each dot represents one gene. The x-axis variable is the mean log2 fold change in the five nonhematopoietic cells (endothelial cells, fibroblasts, myoblasts, osteoblasts, and preadipocytes), and the y-axis variable is the mean log2 fold change (FC) in the four hematopoietic cells (B cells, CD4 + T cells, monocytes, and neutrophils). The four tails of the distribution are color-coded and represent genes with evidence of transcriptional response to glucocorticoid (defined here as a mean log2 fold change ≥ 0.5 or ≤ −0.5) in one group of cells but not in the other. The right panel displays the baseline expression levels in hematopoietic versus nonhematopoietic cells for the genes with strongest evidence of a transcriptional response to glucocorticoid in one group of cells but not in the other (genes at the four tails of the distribution, as defined above). The values displayed are the mean log2 normalized read count at baseline in nonhematopoietic cells (x axis) versus hematopoietic cells (y axis). (b) Transcriptional response of TRIM22 to in vitro glucocorticoid treatment in nine primary human cell types. (c) Transcriptional response of ITGA5 to in vitro glucocorticoid treatment in nine primary human cell types. In b and c, the values displayed are the normalized read counts in vehicle-treated cells (VH; average of 2 and 6 h) and in glucocorticoid-treated cells (GC; 2 or 6 h). Each dot represents one biological replicate (one donor). Multiple-testing-adjusted P values ( q ) are from comparisons of glucocorticoid-treated versus vehicle-treated cells at each of the two time points. ns, not significant ( q > 0.05).

Article Snippet: Human primary osteoblasts from adult (Lonza; cat. no. CC-2538, lot no. 0000435102) or child (Lonza; cat. no. CC-2538, lot nos.

Techniques: Cell Culture, Purification, In Vitro, RNA Sequencing, Quantitative Proteomics, Dispersion, Expressing

Journal: Bone

Article Title: Ex Vivo Construction of Human Primary 3D-Networked Osteocytes

doi: 10.1016/j.bone.2017.09.012

Figure Lengend Snippet: [32] (a) microfluidic perfusion device with 6 culture chambers, (b) cross-sectional view of a 3D culture chamber with the red arrows indicating the overall direction of culture medium flow through the device, (c) schematic illustration of microbeads-guided assembly, and (d) histologic image of 3D-networked osteocytes with the red arrows indicating medium flow direction with respect to the tissue sample. Scale bar: 20 µm.

Article Snippet: A human 3D bone tissue model was developed by constructing ex vivo the 3D network of osteocytes via: (1) the biomimetic assembly of primary human osteoblastic cells with 20–25 μm and BCP microbeads and (2) subsequent microfluidic perfusion culture.

Techniques:

Journal: Bone

Article Title: Ex Vivo Construction of Human Primary 3D-Networked Osteocytes

doi: 10.1016/j.bone.2017.09.012

Figure Lengend Snippet: (a) hip fragment shown as an example; (b) as-isolated cells after 4 collagenase digestion cycles; (c) proliferated osteoblastic cells after 10 days of 2D culture; (d) 3D tissue sample constructed using 20–25 µm microbeads and proliferated cells and 14 days of perfusion culture; (e) H&E histologic images showing the formation of 3D cellular network as indicated by black arrows in (f) and white arrows in (g); and (h) immunostaining for sclerostin (red). (d) –(f) from patient sample #6 and (g)–(h) from patient sample #4. Scale bar: 25 µm.

Article Snippet: A human 3D bone tissue model was developed by constructing ex vivo the 3D network of osteocytes via: (1) the biomimetic assembly of primary human osteoblastic cells with 20–25 μm and BCP microbeads and (2) subsequent microfluidic perfusion culture.

Techniques: Isolation, Construct, Immunostaining

Journal: Clinical Oral Investigations

Article Title: Backscatter from therapeutic doses of ionizing irradiation does not impair cell migration on titanium implants in vitro

doi: 10.1007/s00784-023-05128-6

Figure Lengend Snippet: Relative DNA damage with %DNA in tail normalized to the unirradiated samples on corresponding surface (C − ) and positive TCP control (C + ; 3% H 2 O 2 ), of primary human osteoblasts (OBs) and human mesenchymal stem cells (MSCs) immediately after irradiation with 0, 2, 6, and 10 Gy, while growing on tissue culture polystyrene (TCP), minimally rough machined titanium (Ti), or moderately rough fluoride-modified titanium (TiF) ( n = 3). Data are presented as mean ± SEM. * p ≤ 0.05 compared to corresponding 0 Gy control (C. − )

Article Snippet: Commercially available primary human osteoblasts (OBs; batch#: 0000426160) and human mesenchymal stem cells (MSCs; batch#: 0000684888) (Lonza, Walkersville, MD, USA) were routinely cultured at 37 °C/5% CO 2 in osteoblast basal medium (C-27010) supplemented with Osteoblast Growth Medium SupplementMix (C-39615) (Promocell, Heidelberg, Germany), or in human Mesenchymal Stem Cell Growth BulletKit Medium (MSCGM catalog no. PT-3001) (Lonza) respectively, both containing L-glutamine, gentamicin sulfate-amphotericin (GA), and 10% fetal bovine serum (FBS).

Techniques: Control, Irradiation, Modification

Journal: Clinical Oral Investigations

Article Title: Backscatter from therapeutic doses of ionizing irradiation does not impair cell migration on titanium implants in vitro

doi: 10.1007/s00784-023-05128-6

Figure Lengend Snippet: Representative images of primary human osteoblasts (OBs) migration and gap closure (GC) on tissue culture polystyrene (TCP), minimally rough machined titanium (Ti), and moderately rough fluoride-modified titanium (TiF), after no irradiation and 14 Gy (TCP) or 10 Gy (Ti + TiF), at the timepoints 0, 24, 48, and 72 h. As full gap closure was already observed at 48 h for all TCP samples, no imaging was performed at later timepoints. Scale bar: 200 µm

Article Snippet: Commercially available primary human osteoblasts (OBs; batch#: 0000426160) and human mesenchymal stem cells (MSCs; batch#: 0000684888) (Lonza, Walkersville, MD, USA) were routinely cultured at 37 °C/5% CO 2 in osteoblast basal medium (C-27010) supplemented with Osteoblast Growth Medium SupplementMix (C-39615) (Promocell, Heidelberg, Germany), or in human Mesenchymal Stem Cell Growth BulletKit Medium (MSCGM catalog no. PT-3001) (Lonza) respectively, both containing L-glutamine, gentamicin sulfate-amphotericin (GA), and 10% fetal bovine serum (FBS).

Techniques: Migration, Modification, Irradiation, Imaging

Journal: Clinical Orthopaedics and Related Research

Article Title: Antibacterial and Biocompatible Titanium-Copper Oxide Coating May Be a Potential Strategy to Reduce Periprosthetic Infection: An In Vitro Study

doi: 10.1007/s11999-016-4713-7

Figure Lengend Snippet: (A) Normal human osteoblast cells (Lonza) were separately seeded in 12-well plates (Corning) at a concentration of 3 × 104 cells per well. OGM medium (Lonza), a cell culture medium optimized for osteoid cell lineages, was used to feed the cells. Once cells reached 90% confluence, a Netwell® insert (Corning) was placed in each well to suspend the discs and avoid direct contact with the cells (black arrow). Two milliliters of culture medium were needed to completely cover the discs. To simulate normal extracellular fluid turnover, cell medium was exchanged every 24 hours. (B) Side view of 12-well plates (Corning). Black arrow is pointing how the cells are separated from the discs.

Article Snippet: Normal Human Osteoblast Viability Normal human osteoblast cell lines (Lonza, Allendale, NJ, USA) were used for cell viability experiments.

Techniques: Concentration Assay, Cell Culture

Journal: Clinical Orthopaedics and Related Research

Article Title: Antibacterial and Biocompatible Titanium-Copper Oxide Coating May Be a Potential Strategy to Reduce Periprosthetic Infection: An In Vitro Study

doi: 10.1007/s11999-016-4713-7

Figure Lengend Snippet: These graphs compare mean and SD of relative cell viability of normal human osteoblasts (Normal human osteoblast) at 24 hours (A) and Day 7 (B) after of exposure using annexin/PI staining.

Article Snippet: Normal Human Osteoblast Viability Normal human osteoblast cell lines (Lonza, Allendale, NJ, USA) were used for cell viability experiments.

Techniques: Staining

Journal: Clinical Orthopaedics and Related Research

Article Title: Antibacterial and Biocompatible Titanium-Copper Oxide Coating May Be a Potential Strategy to Reduce Periprosthetic Infection: An In Vitro Study

doi: 10.1007/s11999-016-4713-7

Figure Lengend Snippet: Summary of antibacterial, cell viability, and elution characteristics of different coating compositions at 24 hours

Article Snippet: Normal Human Osteoblast Viability Normal human osteoblast cell lines (Lonza, Allendale, NJ, USA) were used for cell viability experiments.

Techniques: